label it sirna localization kit cy3  (Mirus Bio)

Journal: Nucleic Acid Therapeutics

Article Title: Fluorogenic RNA Nanoparticles for Monitoring RNA Folding and Degradation in Real Time in Living Cells

doi: 10.1089/nat.2012.0380

Figure Lengend Snippet: White light and fluorescence images of KB cells with and without electroporation of either 0.5 μM Cy3-labeled pRNA-3WJ-(MGapt) or 5 μM pRNA-3WJ-(MGapt) in the presence of 10 μM MG. The MG aptamer fluorescence can be clearly visualized in the pRNA-3WJ-(MGapt) electroporated sample. As a positive control for electroporation Cy3- pRNA-3WJ-(MGapt) can be seen inside of the cells with electroporation but not in the non-electroporated sample.

Article Snippet: For fluorescence labeling of the RNA strands, the fluorescent dye Cy3 was labeled on the pRNA-3WJ-(MGapt) using the Label IT siRNA Tracker Intracellular Localization Kit, Cy3 ™ (Mirus Bio LLC).

Techniques: Fluorescence, Electroporation, Labeling, Positive Control

Journal: Nature nanotechnology

Article Title: Thermodynamically Stable RNA three-way junctions as platform for constructing multi-functional nanoparticles for delivery of therapeutics

doi: 10.1038/nnano.2011.105

Figure Lengend Snippet: a, Temperature effects on the stability of 3WJ-pRNA core, denoted [ab*c] 3WJ evaluated by 16% native gel. Fixed concentration of Cy3 labeled [ab*c] 3WJ was incubated with varying concentration of unlabeled b 3WJ at 25°C, 37°C, and 55°C. b, Urea denaturing effects on the stability of [ab*c] 3WJ evaluated by 16% native gel. Fixed concentration of labeled [ab*c] 3WJ was incubated with unlabelled b 3WJ at ratios of 1:1 and 1:5 in the presence of 0–6M urea at 25°C. c, Dissociation assay for the [ 32 P]-3WJ-pRNA complex harboring three monomeric pRNA by 2-fold serial dilution (lanes 1–9). Monomer unit is shown on the left.

Article Snippet: Fluorescent dyes were labeled on the 106-nt RNA strand by using the Label IT® siRNA Tracker Intracellular Localization Kit, Cy3 ™ (Mirus Bio LLC).

Techniques: Concentration Assay, Labeling, Incubation, Serial Dilution

Journal: Nature nanotechnology

Article Title: Thermodynamically Stable RNA three-way junctions as platform for constructing multi-functional nanoparticles for delivery of therapeutics

doi: 10.1038/nnano.2011.105

Figure Lengend Snippet: a, Flow cytometry revealed the binding and specific entry of fluorescent-[3WJ-pRNA-siSur-rZ-FA] nanoparticles into folate receptor positive (FA + ) cells. Positive and negative controls were Cy3-FA-DNA and Cy3-[3WJ-pRNA-siSur-rZ-NH 2 ] (without FA), respectively. b, Confocal images showed targeting of FA + -KB cells by co-localization (overlap, 4) of cytoplasma (green, 1) and RNA nanoparticles (red, 2) (magnified, bottom panel). Blue–nuclei, 3. c–d , Target gene knock-down effects showed by (c) qRT-PCR with GADPH as endogenous control and by (d) Western blot assay with β–actin as endogenous control. e, 3WJ-pRNA nanoparticles target FA + tumor xenografts upon systemic administration in nude mice. Upper panel: whole body; Lower panel: organ imaging; Lv=liver; K=kidney; H=heart; L=lung; S=spleen; I=intestine; M=muscle; T=tumor). Scale: Fluorescent Intensity.

Article Snippet: Fluorescent dyes were labeled on the 106-nt RNA strand by using the Label IT® siRNA Tracker Intracellular Localization Kit, Cy3 ™ (Mirus Bio LLC).

Techniques: Flow Cytometry, Binding Assay, Quantitative RT-PCR, Western Blot, Imaging